There are many methods to detect HCG. At present, immunological methods are mainly used in clinical practice, such as monoclonal antibody colloidal gold test, ELISA method, electrochemiluminescence method, radioimmunoassay, pregnancy card method, latex agglutination inhibition test, hemagglutination inhibition test, etc. . The immunological method is simple, fast, and highly sensitive, and its detection methods are constantly updated, and it is developing in the direction of specificity, speed, simplicity, sensitivity, and popularity. Biological testing is the main method for early detection of HCG.
In the monoclonal antibody colloidal gold test, the test strip is immersed in the urine to be tested (the liquid level should be lower than two antibody lines) and then quickly taken out. The urine continues upward along the test strip, and the β-hCG in the urine is upward. In the process, it is combined with the gold-labeled β-hCG monoclonal antibody. When the goat anti-human HCG antibody line is reached, the gold-labeled β-hCG monoclonal antibody-urine HCG-goat anti-human HCG polyclonal antibody complex is formed, which is on the test strip A magenta line is displayed. Result judgment: Red at the quality control point and measurement point is positive; only red at the quality control point is negative. If the quality control point and the measurement point are not red, it means that the reagent is invalid. The monoclonal antibody colloidal gold detection kit has membrane percolation method (showing 2 red spots) and test strip method (showing 2 red bars), the sensitivity is about 0.8～2.0ng/L, semi-quantitative, and widely used in clinical practice And family.
